Reverse vaccinology pipeline for epitope-based vaccine development and its application in human leishmaniases

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Pissarra, Joana; Petitdidier, Elodie; Pagniez, Julie; Meddeb-Garnaoui, Amel; Lemesre, Jean-Loup; Bras-Gonçalves, Rachel, 2026, "Reverse vaccinology pipeline for epitope-based vaccine development and its application in human leishmaniases", https://doi.org/10.23708/0FAK1C, DataSuds, V1

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Description	A computational–experimental pipeline was used to screen the secretomes of six human pathogenic Leishmania species and identify pan-specific T-cell epitopes as potential candidates for a pan-leishmaniasis vaccine. Computationally predicted antigens were experimentally validated using PBMCs from naturally exposed healed human donors. IFN-γ ELISpot and multiplex cytokine bead assays (CBA) revealed positive immune recall responses against several peptide pools, confirming their predicted immunogenicity in vitro. Experimental immunological data were generated from samples collected from healed donors (TUN1,TUN3,TUN4,TUN5,TUN6,TUN7,TUN8,TUN9) previously cured of cutaneous leishmaniasis (CL) caused by L. major in Gafsa, central Tunisia, and from healthy individuals (TUNN1, TUNN2, TUNN3, TUNN4, TUNN5, TUNN6, TUNN7, TUNN8,TUNN9, TUNN10) with no history of leishmaniasis recruited from low- or non-endemic areas (Tunis). (2026-09-14)
Subject	Medicine, Health and Life Sciences
Keyword	Leishmania <genus>, peripheral blood mononuclear cell, Epitopes, T-Lymphocyte, T-helper 1 type immune response, Cytokine, Secretome, Reverse Vaccinology, t cell epitope prediction, MHC protein complex binding, peptide antigen binding, peptide vaccine
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Citation Metadata

Persistent Identifier	doi:10.23708/0FAK1C
Publication Date	2026-10-08
Title	Reverse vaccinology pipeline for epitope-based vaccine development and its application in human leishmaniases
Subtitle	Experimental data generated through immunological analyses (ELISpot Data and Multiplex cytokine-bead assay (CBA)
Alternative Title	Reverse vaccinology pipeline and pan‑leishmania epitope vaccines
Author	Pissarra, Joana (Institut de Recherche pour le Développement) - ORCID: 0000-0002-0506-8042 Petitdidier, Elodie (Institut de Recherche pour le Développement) - ORCID: 0000-0002-8554-8424 Pagniez, Julie (Institut de Recherche pour le Développement) - ORCID: 0000-0002-2151-3572 Meddeb-Garnaoui, Amel (Institut Pasteur de Tunis) - ORCID: 0000-0002-1581-2779 Lemesre, Jean-Loup (Institut de Recherche pour le Développement) - ORCID: 0000-0002-7406-8820 Bras-Gonçalves, Rachel (Institut de recherche pour le développement) - ORCID: 0000-0002-3089-4530
Point of Contact	Use email button above to contact. Pissarra, Joana (IRD - Institut de Recherche pour le Développement) Pagniez, Julie (Institut de Recherche pour le Développement) Bras-Gonçalves, Rachel (Institut de recherche pour le développement)
Description	A computational–experimental pipeline was used to screen the secretomes of six human pathogenic Leishmania species and identify pan-specific T-cell epitopes as potential candidates for a pan-leishmaniasis vaccine. Computationally predicted antigens were experimentally validated using PBMCs from naturally exposed healed human donors. IFN-γ ELISpot and multiplex cytokine bead assays (CBA) revealed positive immune recall responses against several peptide pools, confirming their predicted immunogenicity in vitro. Experimental immunological data were generated from samples collected from healed donors (TUN1,TUN3,TUN4,TUN5,TUN6,TUN7,TUN8,TUN9) previously cured of cutaneous leishmaniasis (CL) caused by L. major in Gafsa, central Tunisia, and from healthy individuals (TUNN1, TUNN2, TUNN3, TUNN4, TUNN5, TUNN6, TUNN7, TUNN8,TUNN9, TUNN10) with no history of leishmaniasis recruited from low- or non-endemic areas (Tunis). (2026-09-14)
Subject	Medicine, Health and Life Sciences
Keyword	Leishmania http://purl.obolibrary.org/obo/NCBITaxon_5658 (NCBI Taxonomy (NCBITaxon)) http://purl.obolibrary.org/obo/ncbitaxon.owl peripheral blood mononuclear cell http://purl.obolibrary.org/obo/CL_2000001 (Cell Ontology (CL)) http://purl.obolibrary.org/obo/cl.owl Epitopes, T-Lymphocyte http://id.nlm.nih.gov/mesh/D018984 (Medical Subject Headings (MeSH)) https://id.nlm.nih.gov/mesh/ T-helper 1 type immune response http://purl.obolibrary.org/obo/GO_0042088 (Gene Ontology (GO)) http://purl.obolibrary.org/obo/go.owl Cytokine http://purl.obolibrary.org/obo/NCIT_C20464 (NCI Thesaurus (NCIt)) http://purl.obolibrary.org/obo/ncit.owl Secretome http://id.nlm.nih.gov/mesh/D000089282 (Medical Subject Headings (MeSH)) https://id.nlm.nih.gov/mesh/ Reverse Vaccinology http://id.nlm.nih.gov/mesh/D000099138 (Medical Subject Headings (MeSH)) https://id.nlm.nih.gov/mesh/ t cell epitope prediction http://purl.obolibrary.org/obo/OBI_0200060 (Ontology for Biomedical Investigations (OBI)) http://purl.obolibrary.org/obo/obi.owl MHC protein complex binding http://purl.obolibrary.org/obo/GO_0023023 (Gene Ontology (GO)) http://purl.obolibrary.org/obo/go.owl peptide antigen binding http://purl.obolibrary.org/obo/GO_0042605 (Gene Ontology (GO)) http://purl.obolibrary.org/obo/go.owl peptide vaccine http://purl.obolibrary.org/obo/VO_0000577 (Vaccine Ontology (VO)) http://purl.obolibrary.org/obo/vo.owl
Scientific Theme	Health: generalities (NumeriSud) https://uri.ird.fr/so/kos/tnu/050
Depositor	PAGNIEZ, Julie
Deposit Date	2026-09-14
Data Type	Experimental data; Aggregate data
Series	ELISpot Dataset: IFN-γ ELISpot PBMCs were cultured for 16–20 h with positive controls (PHA, L.infantum TSLA, LiESAP mL (Leishmania infantum excreted-secreted antigens, GMP-produced CaniLeish® without adjuvant)), peptide pools (ALL_I, ALL_I+II, ALL_II, I_Pool1 at I_Pool7, I_PoolA at I_PoolG, II_Pool1 at II_Pool5, II_PoolA at II_PoolE), or culture medium as a negative control (NS), in triplicate. IFN-γ secretion was detected using a biotinylated anti-human IFN-γ mAb (7-B6-1; Mabtech), and spots were quantified with a CTL ImmunoSpot reader (CTL Analyzers, Shaker Heights, OH, USA). CBA dataset: Multiplex cytokine-bead assay (CBA) Cytokine production was assessed using the LEGENDplex™ Human CD8/NK Panel (V02) (BioLegend). PBMC culture supernatants were analyzed for GM-CSF, Granzyme B, IFN-γ, IL-2, IL-4, IL-6, IL-10, IL-17A, IL-21, MCP-1 (CCL2), Perforin, and TNF-α. Samples were acquired on an Attune Acoustic Focusing Cytometer (Thermo Fisher Scientific), and cytokine concentrations were calculated from fluorescence signals using standard curves generated from the provided cytokine standards.
Software	LEGENDplex, Version: v8.0

Geospatial Metadata

Geographic Coverage	Tunisia Gafsa Tunisia Tunis Governorate
Geographic Bounding Box	8.735689144669793 8.832644855330207 34.464968649311864 34.38503135068813

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