Replication data and R code for: Non-contact detection of pyrethroids widely used in vector control by Anopheles mosquitoes

Version 1.0
Kambou, Sassan Simplice; Valente, Adeline; Agnew, Philip; Hien, Domonbabele Francois De Sales; Yerbanga, Rakiszendé Serge; Moiroux, Nicolas; Dabire, Kounbor Roch; Pennetier, Cedric; Cohuet, Anna; Carrasco, David, 2024, "Replication data and R code for: Non-contact detection of pyrethroids widely used in vector control by Anopheles mosquitoes", https://doi.org/10.23708/IMJ1WG, DataSuds, V1
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Description
Pyrethroids are the most widely used insecticides to control vector borne diseases including malaria. Physiological resistance mechanisms to these insecticides have been well described, whereas those for behavioral resistance remain overlooked. Field data suggest the presence of spatial sensory detection by Anopheles mosquitoes of the pyrethroid molecules used in insecticide-based control tools, such as long-lasting insecticide nets or insecticide residual spraying, opening the way to the emergence of a wide range of behavioral adaptations among malaria vectors. However, the spatial sensory detection of these molecules is controversial and needs to be demonstrated. The goal of this study was to behaviorally characterize the non-contact detection of three of the most common pyrethroids used for malaria vector control: permethrin, deltamethrin an ⍺-cypermethrin. To reach this goal, we recorded the behavior (takeoff response) of Anopheles gambiae pyrethroid-sensitive and resistant laboratory strains, as well as field collected mosquitoes from the Gambiae complex, when exposed to the headspace of bottles containing different doses of the insecticides at 25 and 35°C, in order to represent a range of laboratory and field temperatures.

We found the proportion of laboratory susceptible and resistant female mosquitoes that took off was, in all treatments, dose and the temperature dependent. Sensitive mosquitoes were significantly more prone to take off only in the presence of ⍺-cypermethrin, whereas sensitive and resistant mosquitoes showed similar responses to permethrin and deltamethrin. Field- collected mosquitoes of the Gambiae complex were also responsive to permethrin, independently of the species identity (An. gambiae, An. coluzzi and An. arabiensis) or their genotypes for the kdr mutation, known to confer resistance to pyrethroids. The observed ability of Anopheles spp. mosquitoes to detect insecticides without contact could favor the evolution of behavioral modifications that may allow them to avoid or reduce the adverse effect of insecticides and thus, the development of behavioral resistance.

The files in this repository contain the raw data generated during the experiments and the R script used to analyse the data.
(2024-05-24)
Subject Medicine, Health and Life Sciences
Keyword Spatial detection, pyrethroids, takeoff response, Anopheles mosquitoes
Related Publication Kambou SS, Valente A, Agnew P, Hien DFdS, Yerganga RS, Moiroux N, Dabire KR, Pennetier C, Cohuet A, Carrasco D (2024) Non-contact detection of pyrethroids widely used in vector control by Anopheles mosquitoes. bioRxiv 2024.01.28.577675 doi: 10.1101/2024.01.28.577675
Notes The data are on the number of female mosquitoes responding, or not responding, to the experimental stimuli in each replicate of each treatment, along with the derived statistics, e.g., proportion responding, used in the subsequent analyses.

Data Type: Experimental data
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0_ReadMe.txt
pyrethroids_vector_control_Anopheles_mosquitoes_2021_2022/Plain Text - 2.8 KB
Published Jun 24, 2024
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MD5: f82cd8442b59b02eda7d054223ca1de5
ReadMe file - data citation, description and terms of use.
Documentation
Kambou_et_al_insecticide_data.txt
pyrethroids_vector_control_Anopheles_mosquitoes_2021_2022/Plain Text - 22.0 KB
Published Jun 24, 2024
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MD5: 6b4088edd47f217e807617cabe22f82a
Text file containing raw data for the number of female mosquitoes responding, or not responding, to the experimental stimuli in each replicate of each treatment.
Data
Kambou_et_al_r_code.txt
pyrethroids_vector_control_Anopheles_mosquitoes_2021_2022/Plain Text - 19.8 KB
Published Jun 24, 2024
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MD5: a98a7b4cbd30efb3f10069df97034207
Text file containing notes & R script to analyse the accompanying data
Code
Kambou_et_al_variables_data_dictionary.txt
pyrethroids_vector_control_Anopheles_mosquitoes_2021_2022/Plain Text - 1.6 KB
Published Jun 24, 2024
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MD5: 5fe23977159c2c7cf3a3fcb68d5ba7cc
Description of the variables in 'Kambou_et_al_insecticide_data.txt'
Documentation
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