Trypanosoma cruzi cryobank of living isolates and associated metadata for Chagas disease research

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Manzanilla, Vincent; Waleckx, Etienne; Jenny Telleria, 2026, "Trypanosoma cruzi cryobank of living isolates and associated metadata for Chagas disease research", https://doi.org/10.23708/IX0ISV, DataSuds, V1
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Description

The Trypanosoma cruzi cryobank is a curated collection of viable parasite stabilates preserved at −150 °C to ensure long-term conservation of biological material for Chagas disease research. Each record corresponds to a unique isolate or biological clone (when applicable) linked to a documented chain of custody from field or laboratory collection through isolation, stabilisation, storage, and subsequent withdrawals.

The Trypanosoma cruzi cryobank is documented through a harmonised metadata structure designed to ensure traceability, scientific usability, and long-term conservation of parasite diversity. For each isolate or biological clone, the database records a stable internal identifier (Tri), laboratory code, isolate name, species (T. cruzi), and DTU assignment (TcI–TcVI when available), together with associated references or source publications. Geographical provenance is described at multiple administrative levels (country, département, province, communauté/locality) and includes information on ecological origin (domestic, peridomestic, sylvatic or mixed transmission context).

The host and epidemiological context are specified through host species, taxonomic order, common name, transmission cycle classification, and date of parasite isolation. Laboratory-related information includes current culture status and available biological characterisation data (e.g., molecular typing markers, sequencing accession numbers, or relevant phenotypic traits). Cryopreservation metadata document the date of stabilisation, core elements of the cryopreservation protocol (cryoprotectant, freezing procedure, storage at −150 °C), and precise physical storage coordinates within the freezer system (rack, box, row, column). Stock verification and quality control status are recorded to ensure traceability and integrity of stored material.

The cryobank is designed to:

  • Preserve the genetic and phenotypic diversity of T. cruzi across geographic regions, host species, and transmission cycles.
  • Provide reference biological material for experimental research, method validation, and comparative studies.
  • Enable controlled access to well-documented parasite resources under appropriate biosafety, ethical, and material transfer frameworks.

The cryobank operates in accordance with applicable access and benefit-sharing (ABS) regulations, including the Nagoya Protocol.

(2026-01-27)
Subject Medicine, Health and Life Sciences
Keyword Trypanosoma cruzi, Scientific host name, Standardised vernacular name, parasite isolate population, Living Specimen, Parasite cryobank, Parasitology, Chagas disease
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Database_cryobank.csv
Comma Separated Values - 375.2 KB
Published Mar 9, 2026
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MD5: aba5fe17fc16f4de567c37f0a4394445
This table file contains the complete structured metadata associated with the Trypanosoma cruzi cryobank. Each row corresponds to a unique parasite isolate or biological clone preserved at −150 °C, and each column represents a standardised metadata field describing taxonomic identity, provenance, host context, laboratory history, biological characterisation, and storage information. The table includes core identifiers (internal ID, isolate code, species, DTU classification, reference source), detailed geographic provenance (country, administrative subdivisions, locality, ecological origin), host and transmission context (host species, taxonomic order, common name, transmission cycle, date of isolation), laboratory and biological information (culture status, molecular characterisation when available, relevant notes), and cryopreservation metadata (date of stabilisation, storage protocol, and precise physical freezer coordinates such as rack, box, row, and column). A verification field documents stock validation and quality control status.
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Database_cryobank.ods
OpenOffice Spreadsheet - 179.1 KB
Published Mar 9, 2026
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MD5: 0ca5b5aab715306ea1d9f2febb3e7f1c
Description of the table file This table file contains the complete structured metadata associated with the Trypanosoma cruzi cryobank. Each row corresponds to a unique parasite isolate or biological clone preserved at −150 °C, and each column represents a standardised metadata field describing taxonomic identity, provenance, host context, laboratory history, biological characterisation, and storage information. The table includes core identifiers (internal ID, isolate code, species, DTU classification, reference source), detailed geographic provenance (country, administrative subdivisions, locality, ecological origin), host and transmission context (host species, taxonomic order, common name, transmission cycle, date of isolation), laboratory and biological information (culture status, molecular characterisation when available, relevant notes), and cryopreservation metadata (date of stabilisation, storage protocol, and precise physical freezer coordinates such as rack, box, row, and column). A verification field documents stock validation and quality control status.
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README.txt
Plain Text - 1.5 KB
Published Mar 9, 2026
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MD5: 01d764af88fc805375b4b17b94bbbb88
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