Notes
| De novo plastid genome reconstruction was done by mapping raw reads to the C. arabica chloroplast using Bowtie2 (Langmead and Salzberg, 2012), with flags “--very-fast-local” and “--al-conc”. Redundant reads were eliminated using function “clumpify” from the package bbmap (https://github.com/BioInfoTools/BBMap), with the “dedupe” option activated. Finally, the obtained reads were assembled using NOVOPlasty (version 2.7.2) (Dierckxsens, 2017), with a sequence seed corresponding to position 20000 to 20101 to the C. arabica chloroplast. Plastid genomes annotation were done using GeSeq (Tillich et al., 2017). |